TABLE 1.

Evaluation of sensitivity and specificity of three ELISAs on animals at 13 serovar Dublin-infected and 8 control dairy farms

ELISA	Cutoff value	serovar Dublin-infected farms				Control farms (fecal culture-negative animals [n = 840])
		All animals (n = 1,763)		Fecal culture-positive animalsa(n = 50)
		No.b	%b	No.b	%b	No.c	%c
LPS	25	837	47.5	31	62.0	702	83.6
	50	311	17.6	22	44.0	805	95.8
	100	144	8.2	16	32.0	834	99.3
	200	81	4.6	13	26.0	839	99.9
	400	49	2.8	10	20.0	840	100.0
	800	25	1.4	7	14.0	840	100.0
	1,600	14	0.8	6	12.0	840	100.0
	3,200	7	0.4	3	6.0	840	100.0
GP	25	1136	64.4	36	72.0	669	79.6
	50	391	22.2	26	52.0	827	98.5
	100	175	9.9	23	46.0	840	100.0
	200	102	5.8	17	34.0	840	100.0
	400	64	3.6	12	24.0	840	100.0
	800	41	2.3	12	24.0	840	100.0
	1,600	25	1.4	9	18.0	840	100.0
	3,200	9	0.5	4	8.0	840	100.0
GM-DAS	10	135	7.9	19	38.0	840	100.0
	20	92	5.2	18	36.0	840	100.0
	40	61	3.5	15	30.0	840	100.0
	80	46	2.6	12	24.0	840	100.0
	160	26	1.5	7	14.0	840	100.0
	320	12	0.7	4	8.0	840	100.0
	640	7	0.4	3	6.0	840	100.0

^aPresented serology is from the moment feces were culture positive for serovar Dublin for the first time. 

^bPositive at the cutoff titer indicated or at a higher titer. 

^cNegative at a titer lower than the indicated cutoff titer.