Table 2.
Substrate affinity of amidase from Bacillus smithii IIIMB2907 [enzyme assay conditions: substrate concentration: 20 mM reaction temperature: 50 °C, pH 7.0, and incubation time: 30 min]
| Substrates | ≈Solubility in water (mg/mL) | Structure | Activity (U/mL) |
|---|---|---|---|
| Acrylamide | 33.3 |
|
40.58 ± 2.03 |
| Acetamide | 2000 |
|
34.00 ± 1.70 |
| Benzamide | 13 |
|
33.67 ± 1.68 |
| Butyramide | 170 |
|
45.00 ± 2.25 |
| Nicotinamide | 100 |
|
65.63 ± 3.28 |
| Propionamide | 360 |
|
38.00 ± 1.90 |
| Adipamide | 4.4 |
|
29.38 ± 1.47 |
| Malonamide | 180 |
|
6.08 ± 0.30 |
| Succinamide | 3.30 |
|
11.54 ± 0.58 |
| Valeramide | 92 |
|
34.33 ± 1.72 |
| Pyrazinamide | 50 |
|
66.17 ± 3.31 |
| Isonicotinamide | 191 |
|
68.21 ± 3.41 |
| Isobutyramide | 225 |
|
30.17 ± 1.51 |
| Methacrylamide | 202 |
|
27.50 ± 1.38 |