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. Author manuscript; available in PMC: 2022 Jan 16.
Published in final edited form as: Methods Mol Biol. 2019;1937:155–175. doi: 10.1007/978-1-4939-9065-8_9

Fig. 2. Generation of AdV vector using Cre/loxP recombination system.

Fig. 2.

Co-transfection of pAdVΔψ,E1,E3/loxP and pAdVshuttle/loxP (AdV vector shuttle plasmid for cre/loxP recombinase system) containing the desired transgene, into a cell line expressing Cre recombinase protein and E1 proteins of AdV, AdV vector expressing the inserted transgene protein or peptide will be generated.

AdV, adenovirus; R-ITR, right inverted terminal repeats of adenovirus genome; L-ITR, left inverted terminal repeats of adenovirus genome; CMV, cytomegalovirus promoter; PolyA, polyadenylation signal; AmpR, ampicillin resistance gene; Ori, plasmid bacterial origin of replication; ΔE1, Adenovirus genome without the E1 region; ΔE3, Adenovirus genome without the E1 region; ψ, AdV packaging signal.