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. Author manuscript; available in PMC: 2022 Nov 20.
Published in final edited form as: Free Radic Biol Med. 2021 Sep 20;176:92–104. doi: 10.1016/j.freeradbiomed.2021.09.013

Figure 8.

Figure 8.

Treatment with Ferrostatin-1 mitigates the negative effects of H114 expression on cell viability and growth. A. The effect of Ferrostatin-1 treatment (2μM) on cell viability of Doxycycline (DOX; 0, 0.5, 1.0, 1.5, 2.0, 2.5 μg/ml)-treated Control (Upper), CISD2 (Middle) and H114C (Lower) cells at 72 hours following DOX application (with or without Ferrostatin). Cell viability was measured using Alamar Blue. B. The effect of Ferrostatin-1 treatment (2μM) on cell growth of Doxycycline (DOX; 0, 0.5, 1.0, 1.5, 2.0, 2.5 μg/ml)-treated Control (Upper), CISD2 (Middle) and H114C (Lower) cells at 72 hours following DOX application (with or without Ferrostatin). Cell growth was measured with an Incucyte® Live-Cell Imaging apparatus. Results in A and B are shown as mean ± SE of 7 biological control averages, each conducted with 3 technical repeats. N=7; two-way ANOVA followed by a Tukey test; ns, not significant.