Fig. 3.

Immunohistochemical images for Rb phosphorylation and E2F1 expression and securin gene structure. Immunohistochemical assays of A phosphor-retinoblastoma (pRb) and C E2F1 expression in mouse stomach cells with and without RAN + lime treatment. Magnification (100x) of the marked area is shown. Grouped scatterplot illustrating quantitative values within each grouping of H-scores which are represented in B and D as the mean ± SEM; P-values were calculated with untreated control using one-way ANOVA with Tukey’s multiple comparison post-tests. In each case images of two mice were shown. The scale bar: 200 µm. E Schematic illustration of the mouse PTTG1/securin gene structure. Exons are indicated as boxes (translated sequences in black and untranslated regions in white). Exon and intron sizes (bp) are indicated. The transcription initiation site (TSS) is shown as + 1 and the translation start site ATG is indicated with an arrow. The promoter region is shown in Kb and the region is highlighted from where the primer pair was designed. The lower panel represents cDNA with a scale multiple of 100 bp. Both 5`- and 3`-flanking untranslated regions are shown