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. Author manuscript; available in PMC: 2022 Jan 16.
Published in final edited form as: Cell Rep. 2021 Nov 16;37(7):110013. doi: 10.1016/j.celrep.2021.110013

Figure 1. LPA and ATX/LPC are chemorepulsive for TILs and peripheral CD8+ T cells.

Figure 1.

(A) Transwell migration of ex vivo expanded TILs from two melanoma patients stimulated with LPA(18:1) at the indicated concentrations. Chemokine CXCL10 (1 μM) was used as positive control; “control” refers to serum-free medium. Agonists were added to the bottom wells and incubation was carried out for 2 h at 37°C.

(B) LPA dose-dependency of migration. The inset shows a representative transwell filter after staining. Migration was quantified by color intensity using ImageJ.

(C) LPA overrules CXCL10-induced TIL chemotaxis. LPA(18:1) was added together with CXCL10 at the indicated concentrations.

(D) Migration of CD8+ T cells isolated from peripheral blood, measured in the absence (control) and presence of the indicated concentrations of LPA(18:1). Note that the presence of 0.5% serum has no effect.

(E) Recombinant ATX (20 nM) added together with the indicated concentrations of LPC(18:1) recapitulates the inhibitory effects of LPA(18:1) on TILs and CD8+ T cells.

(A and C–E) Results are representative of three independent experiments each performed in technical triplicates and expressed as means ± SEM; bars annotated with different letters were significantly different according to Fisher’s least significant difference (LSD) test (p ≤ 0.05) after ANOVA.