Figure 6. Enforced ATX expression in tumor cells inhibits infiltration of effector CD8⁺ T cells and impedes vaccine-induced tumor control.

(A–F) Tumor analysis by immunohistochemistry on day 18 in the same mice as analyzed in Figure 5.

(A) Representative heatmaps of CD8⁺ immunostainings of tumor sections from vaccinated mice bearing TC-1^WT or TC-1^ATX tumors.

(B–D) Quantification in percentages of CD8⁺ (B, representative for the data shown in A), CD4⁺ (C), and FOXP3⁺ (D) cells out of all nucleated cells as assessed by immunostaining of tumor sections from vaccinated mice bearing TC-1^WT or TC-1^ATX tumors. Data are depicted as mean + SD, *p < 0.05 (Mann-Whitney U test).

(E–G) TC-1^WT (n = 6) and TC-1^ATX (n = 5) tumor-bearing mice received vaccination as outlined in Figure 5 and tumor growth was monitored over time up to day 70.

(E) Individual growth curves of TC-1^WT and TC-1^ATX tumors in vaccinated mice. Black lines represent group average.

(F) Tumor growth delay following vaccination, expressed as number of days required to reach a tumor size corresponding to that at day 7 (see E). Data are depicted as mean + SD, *p < 0.05 (Mann-Whitney U test).

(G) Overall survival curves of tumor-bearing mice. **p < 0.01 (Mantel-Cox analysis).

Data in this figure are from one experiment representative of two independent experiments.