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. Author manuscript; available in PMC: 2022 Jan 16.
Published in final edited form as: J Am Soc Mass Spectrom. 2020 Jun 30;31(12):2547–2552. doi: 10.1021/jasms.0c00128

Figure 1.

Figure 1.

Elements of the RastirX User Interface.

A) The video camera is calibrated using a precision scale and the calibration line tool. The user draws a line over the image of the scale and enters its length in the Pixel Calibration box. RastirX uses the length of the line in camera pixel units to calculate pixels/mm and mm/pixel. While drawing the calibration line the user can use the zoom and pan tools for the video image to accurately position the endpoints. B) Using the ROI rectangle tool, the user identifies an area of the sample to be imaged (cyan box) and enters dimensional parameters in the Scan Region Identification panel. Either the number of spots and lines or the spot and line spacing can be entered and held constant. RastirX updates the unconstrained quantities as the ROI is drawn or resized.