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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Food Chem Toxicol. 2021 Jun 2;154:112288. doi: 10.1016/j.fct.2021.112288

Fig. 1. Differentiation of hiPSC into cortical glutamatergic neurons.

Fig. 1

hiPSCs were differentiated into cortical glutamatergic neurons for 30 days. β3-tubulin positive neurites and PAX6 positive nuclei are abundant in these cultures (A). In addition, these neurons are also positive for MAP2 and glutamate (B), as well as VGluT1 (C). The temporal expression of the genes PAX6, FOXG1, OTX2 and SOX1 (encode neural precursor cell markers) and TUBB3 and MAP2 (encode neuronal markers) were quantified in day 25 neuronal cultures by RT-qPCR (D). (A-C: scale bars = 50 μm); (D = mean ± 95% confidence intervals, n = 6, technical replicates). Similar gene expression patterns were observed in several other hiPSC lines from control subjects as well as patients with different disease-specific mutations, an additional example of another control line is provided in suppl. Fig. 1.