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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Food Chem Toxicol. 2021 Jun 2;154:112288. doi: 10.1016/j.fct.2021.112288

Table 1. Relative expression of selected genes representing cell type markers across clusters.

Gene expression levels indicated in green (higher) to red (lower) shades are expressed on ‘ScaleData’ units so that the mean expression across cells is 0 and the variance across cells is 1 to provide equal weight to all genes. Key: RG, radial glia; IPC, intermediate progenitor cell; EN, excitatory neuron; CP, choroid plexus progenitor; EP, ependymal; VF, ventral forebrain.

gene_id C0 C1 C2 C3 C4 C5 C6 C7 C8 Marker type Alt Gene Name
PAX6 0.19 0.50 −0.92 −0.79 −0.22 0.16 1.45 −0.62 −1.19 RG
NES 0.43 0.35 −0.82 −0.86 0.85 −0.09 −0.82 −0.83 0.46 RG Nestin
OTX1 0.25 0.22 −0.50 −0.58 −0.26 −0.16 0.14 −0.33 −0.63 RG
SOX1 0.14 0.25 −0.45 −0.38 0.04 0.09 −0.46 −0.47 −0.45 RG
SOX2 0.56 0.65 −1.33 −1.21 0.37 −0.66 −1.21 −1.13 −1.29 RG, EP
GLI3 0.56 0.41 −1.00 −0.93 −0.67 −0.35 −0.43 −0.82 −0.73 RG
GFAP −0.02 0.00 −0.05 −0.05 0.27 −0.05 −0.05 −0.05 −0.05 RG
EMX2 0.30 0.32 −0.91 −0.83 −0.83 −0.26 −1.34 −0.79 −1.33 RG
SLC1A3 0.22 0.19 −0.47 −0.46 0.28 −0.33 −0.43 0.89 1.96 RG
HES1 0.37 0.53 −1.23 −1.20 −0.18 −0.92 −0.56 −0.62 −0.21 RG
VIM 0.56 0.57 −1.48 −1.53 0.52 −0.42 0.66 −1.51 0.63 RG
FOXP1 0.31 0.27 −0.58 −0.61 −0.33 −0.25 0.58 0.16 0.97 RG
MKI67 −0.31 1.25 −0.61 −0.62 −0.30 0.51 −0.37 −0.51 −0.45 RG
DLL1 −0.21 −0.06 0.05 −0.31 −0.29 2.94 −0.35 −0.36 −0.35 IPC
EOMES −0.25 −0.23 0.25 0.02 −0.21 2.68 −0.23 −0.23 −0.27 IPC TBR2
NEUROD1 −0.32 −0.31 1.02 −0.08 −0.31 0.91 −0.30 0.86 −0.33 IPC
NEUROD4 −0.18 −0.18 0.03 −0.18 −0.14 3.40 −0.18 0.16 −0.19 IPC
CCND1 0.42 0.31 −0.98 −0.96 0.77 −0.53 0.93 −0.35 0.23 IPC
NEUROG1 −0.16 −0.14 0.00 −0.15 −0.11 2.69 −0.11 0.59 −0.19 IPC
NEUROG2 −0.17 0.33 0.10 −0.79 −0.79 1.62 −0.79 −0.76 −0.80 IPC
ASCL1 −0.08 0.21 −0.28 −0.20 −0.05 1.42 −0.37 −0.40 −0.38 IPC
NEUROD4 −0.18 −0.18 0.03 −0.18 −0.14 3.40 −0.18 0.16 −0.19 IPC
ASCL1 −0.08 0.21 −0.28 −0.20 −0.05 1.42 −0.37 −0.40 −0.38 IPC
FOXG1 0.04 −0.03 0.57 −0.24 −1.22 −0.20 −1.02 −0.88 −1.44 EN
NEUROD2 −0.42 −0.41 1.76 −0.29 −0.43 −0.23 −0.41 −0.37 −0.44 EN
NEUROD6 −0.47 −0.46 1.78 −0.32 −0.48 0.09 −0.44 −0.41 −0.49 EN
SLC17A6 −0.27 −0.27 0.47 1.48 −0.26 −0.14 −0.28 −0.13 −0.28 EN VGLUT2
SLC17A7 −0.30 −0.29 1.28 −0.23 −0.30 −0.21 −0.30 −0.19 −0.31 EN VGLUT1
MAP2 −0.57 −0.50 1.24 1.31 −0.01 0.31 −1.02 −0.83 −1.10 EN
MAPT −0.45 −0.47 1.03 1.98 −0.45 −0.47 −0.44 0.16 −0.49 EN
TUBB3 −0.29 −0.30 0.98 0.55 −0.46 0.13 −0.61 −0.11 −0.56 EN
DCX −0.57 −0.60 1.41 1.76 −0.39 0.04 −0.73 −0.06 −0.76 EN
NCAM1 −0.34 −0.19 0.70 1.44 −0.73 −0.19 −0.86 −0.07 −0.82 EN
NCAM2 −0.26 −0.24 0.81 0.26 −0.18 −0.22 0.03 −0.01 0.05 EN
FEZF1 −0.13 −0.12 0.14 0.44 0.23 0.16 −0.17 1.21 −0.17 EN
FEZF2 −0.02 0.00 0.33 −1.31 −0.41 0.70 −1.29 −1.32 −1.38 EN
CALB2 −0.30 −0.29 0.63 0.69 −0.26 −0.28 −0.31 1.14 −0.32 EN Calretinin
BCL11B −0.37 −0.32 1.27 0.00 −0.46 −0.10 −0.50 0.27 −0.57 EN
NFIA −0.31 −0.31 1.35 −1.32 0.08 0.18 −0.95 −1.00 −0.95 EN
NFIB −0.27 −0.23 1.33 −1.21 −0.70 0.63 −0.77 −0.09 −0.64 EN
SATB2 0.02 0.01 −0.03 −0.14 −0.05 −0.01 −0.06 0.00 −0.09 EN
SYN1 −0.25 −0.25 0.60 1.16 −0.22 −0.24 −0.21 −0.09 −0.17 EN
HOMER1 −0.18 −0.25 0.38 0.50 0.03 −0.03 0.16 0.02 0.19 EN
TBR1 −0.44 −0.44 1.52 0.48 −0.42 −0.24 −0.42 −0.43 −0.41 EN
DLG4 −0.28 −0.22 0.74 0.53 −0.19 −0.24 −0.20 −0.27 −0.41 EN
MAP2 −0.57 −0.50 1.24 1.31 −0.01 0.31 −1.02 −0.83 −1.10 EN
BDNF −0.07 −0.07 0.00 0.14 0.36 −0.08 0.01 0.29 0.59 EN
DLX2 −0.06 −0.06 −0.03 0.60 −0.07 −0.07 −0.08 0.09 −0.08 VF
FOXP2 −0.05 −0.05 −0.11 0.66 −0.16 −0.09 0.63 0.11 0.44 VF
GBX2 −0.04 −0.04 −0.04 0.09 0.17 0.00 0.04 −0.04 −0.04 VF
PBX3 −0.15 −0.03 −0.42 2.61 −0.19 −0.30 0.14 0.39 −0.07 VF
MEIS2 0.18 0.02 −0.52 1.73 −0.67 −0.63 0.17 0.24 −0.67 VF
PCP4 −0.20 −0.21 −0.10 0.06 2.04 −0.22 0.78 0.32 −0.09 CP
NEAT1 −0.11 −0.20 −0.35 −0.10 1.77 −0.20 4.22 0.81 1.90 CP
RSPO1 −0.17 −0.16 −0.17 −0.16 2.44 −0.16 −0.13 −0.15 −0.17 CP
RSPO2 −0.20 −0.21 −0.21 −0.19 2.90 −0.19 −0.18 −0.21 −0.21 CP
RSPO3 −0.22 −0.29 0.25 −0.15 2.59 −0.28 −0.36 −0.37 −0.34 CP
IGFBP7 −0.19 −0.19 −0.19 −0.19 1.34 −0.19 5.63 0.68 3.23 CP
TTR −0.20 −0.20 −0.18 −0.18 1.57 −0.20 −0.19 −0.24 −0.23 CP
CXCL14 −0.13 −0.10 −0.14 −0.13 1.00 −0.14 1.19 5.73 −0.11 CP
TRPM3 −0.20 −0.20 −0.21 −0.09 1.84 −0.22 4.89 −0.15 −0.20 CP
AQP1 −0.03 −0.04 −0.01 −0.06 0.11 −0.03 0.60 −0.07 −0.07 CP
OTX2 −0.20 −0.19 −0.21 −0.19 2.31 −0.15 4.15 −0.08 −0.22 CP
KCNE2 −0.03 −0.03 0.01 −0.04 0.01 −0.05 0.03 −0.05 −0.05 CP
FOXJ1 −0.03 −0.06 −0.19 −0.20 1.10 −0.13 −0.01 −0.21 −0.21 CP, EP