TABLE 3.

Negative ion nanoES-MS data and proposed compositions of MAb 5F5.9 reactive (738-P) and non-reactive O-deacylated LPS from H. somnus strain 738^a

Strain	Molecular mass (Da)		Relative intensity	Proposed composition
	Observed	Calculated
738 (5F5.9 negative)	2,224.0	2,224.1	0.2	2Hex, 2Hep, PEtn, 2Kdo, lipid A
	2,226.0	2,227.0	0.2	Hex, 2Hep, PEtn, PCho, 2Kdo, lipidA
	2,386.0	2,386.2	0.2	3Hex, 2Hep, PEtn, 2Kdo, lipid A
	2,389.0	2,389.1	0.4	2Hex, 2Hep, PEtn, PCho, 2Kdo, lipid A
	2,590.0	2,589.4	1.0	GlcNAc, 3Hex, 2Hep, PEtn, 2Kdo, lipid A
	2,750.4	2,751.4	0.2	GlcNAc, 4Hex, 2Hep, PEtn, 2Kdo, lipid A
	2,753.4	2,754.5	0.3	GlcNAc, 3Hex, 2Hep, PEtn, PCho, 2Kdo, lipid A
	2,917.0	2,916.7	0.2	GlcNAc, 4Hex, 2Hep, PEtn, PCho, 2Kdo, lipid A
738-P (5F5.9 positive)	2,062.0	2,061.9	1.0	Hex, 2Hep, PEtn, 2Kdo, lipid A
	2,227.4	2,227.0	0.4	Hex, 2Hep, PEtn, PCho, 2Kdo, lipid A

^aAverage mass units were used for calculation of molecular mass based on proposed composition, as follows: Hexose (Hex), 162.15; Heptose (Hep), 192.17; GlcNAc, 203.19; 3-deoxy-d-manno-octulosonic acid (Kdo), 220.18; PEtn, 123.05; PCho, 165.05. A number before an abbreviation signifies the number of components identified.