Figure 2. Distribution of ³²P-labeled FN1040 in the CNS after continuous cerebroventricular administration.

(A) Schema of the brain infusion assembly. An Alzet osmotic pump type 2002 was connected to an indwelling brain infusion cannula with a catheter, which enabled continuous infusion of the aptamer contained in the pump into the lateral cerebroventricle at a flow rate of 1 μl/h for 7 d. (B) Axial sections of the spinal cord (upper row) and the autographic image of corresponding sections (lower row) from the mice infused with ³²P-labeled RNA aptamer for 72 h. The autoradiographic imaging was analyzed by the FUJIFILM Bio-imaging Analyzer BAS-2500. Scale bar = 2 mm. (C) Radioactivity of different brain area analyzed with liquid scintillation counter. Vertical axis is the ratio of counts per minute (CPM) per milligram in each area to total CPM per milligram of four individual experiments. Mean ± SEM. (D) SDS–PAGE analysis of FN1040. Left: Different amounts of unlabeled FN1040 (arrow). Right: autoradiography of SDS–PAGE of tissue homogenate of a mouse infused with ³²P-labeled FN1040 after treatment with proteinase K. F, forebrain; H, hindbrain; M, medulla oblongata; C, cervical cord; T, thoracic cord; L, lumbar cord.

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