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. 2022 Jan 13;5(4):e202101315. doi: 10.26508/lsa.202101315

Figure 6. JNK signaling has broad and profound effects on macrophage polarization.

Figure 6.

(A) Heat map of z-scored phosphosite intensities significantly changing in BMDMs treated with IL4/IL13 or IL4/IL13 + TNF for 0, 30, 60, 120, and 150 min (ANOVA, false discovery rate < 0.05). (B) Heat map of means of z-scored phosphosite intensities significantly changing in BMDMs treated as in (A) and filtered for GOMF “transcription factor binding, transcription factor activity.” (C) Transcription factor enrichment analysis shown as a word cloud where size of name is proportional to the number of gene sets in transcriptional network clusters associated with specific transcription factor. (D) HINT-ATAC analysis of predicted AP-1 binding sites. (E, F) Wild-type BMDMs were stimulated with IL4/IL13, IL4/IL13 + TNF or TNF for 15 min and whole-cell lysates were isolated and analyzed by Western blotting for the indicated proteins. (G) Volcano plots of transcriptomics data set from profile GSE185380. The stimulated groups were compared to the responding Ctrl group. (H) Pdcd1lg2 gene analysis of this data set. All values are means ± SEM; ****P < 0.0001. Statistically significant differences were determined by one-way ANOVA with Tukey correction. Superscripts show statistical significance compared with the control group. n = 3 biological replicates.