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. 2021 Jan 26;29(1):101–121. doi: 10.1038/s41417-021-00293-w

Fig. 4. CircFLNA was high-expressed in lung cancer cells and mainly located in the cytoplasm of SK-MES-1 and A549 cells.

Fig. 4

A The expression level of circFLNA in lung cancer cell lines (NCI-H520, SK-MES-1, H1299, A549, PC-9, and H23) and normal bronchial epithelial cell lines (16HBE and BEAS2B) was determined by RT-qPCR. GAPDH was used as an internal control. All the experiments were conducted three times (*P < 0.05, **P < 0.01, ***P < 0.001, vs. 16HBE; #P < 0.05, ##P < 0.01, ###P < 0.001, vs. BEAS2B). B Cytoplasmic and nuclear RNA fractions were isolated from SK-MES-1 and A549 cells. Relative expression levels of circFLNA and FLNA in the cytoplasm or nucleus were examined by RT-qPCR. GAPDH was used as a cytoplasmic internal control, and U6 was used as a nuclear internal control (^^^P < 0.001, vs. cytoplasm). C The expressions of circFLNA and FLNA in SK-MES-1 and A549 cells treated with or without RNase R were detected by RT-qPCR. GAPDH was used as an internal control (△△△P < 0.001, vs. RNase R−). All experiments were conducted three times.