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. 2021 Jan 26;29(1):101–121. doi: 10.1038/s41417-021-00293-w

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© The Author(s), under exclusive licence to Springer Nature America, Inc. part of Springer Nature 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The expression of miR-486-3p in SK-MES-1 and A549 cells after transfection was detected by RT-qPCR; U6 was used as an internal control. B The viability of SK-MES-1 and A549 cells after transfection was detected by CCK-8 assay. C CCK-8 was used to detect the viability of SK-MES-1 and A549 cells transfected with circFLNA and inhibitor. D The proliferation of SK-MES-1 and A549 cells after transfection was detected by colony formation assay. E The colony formation assay was used to detect the proliferation of SK-MES-1 and A549 cells transfected with circFLNA and inhibitor. F The migration of SK-MES-1 and A549 cells after transfection was detected by wound-healing assay. G The invasion of SK-MES-1 and A549 cells after transfection was detected by transwell assay. All the experiments were conducted three times (^*P < 0.05, ^**P < 0.01, ^***P < 0.001, vs. NC + MC; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs. circ+MC; ^{^}P < 0.05, ^{^^}P < 0.01, ^{^^^}P < 0.001, vs. NC + M). circ circFLNA, NC overexpression negative control, circ circFLNA, M miR-486-3p mimic, MC mimic control.