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. 2021 Jan 26;29(1):101–121. doi: 10.1038/s41417-021-00293-w

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© The Author(s), under exclusive licence to Springer Nature America, Inc. part of Springer Nature 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A The target genes of miR-486-3p were predicted using targetScan and miRWalk. B, C The expressions of WNK2, CYP1A1, PAX5, XRCC1, and TGM3 in SK-MES-1 cells after transfection were detected by western blot. GAPDH was used as an internal control (^***P < 0.001, vs. NC + MC; ^###P < 0.001, vs. circ+MC; ^{^^^}P < 0.001, vs. NC + M). D, E The putative binding site between miR-486-3p and XRCC1 or XRCC1 was predicted using targetScan. F, G The result of Luciferase reporter assay validated that miR-486-3p specifically targeted XRCC1 and CYP1A1 in SK-MES-1 and A549 cells (^△△△P < 0.001, ^△△P < 0.01, vs. NC). H The expressions of XRCC1 and CYP1A1 in the SK-MES-1 cells transfected with circFLNA and mimic were detected by qRT-PCR. I The expression of XRCC1 and CYP1A1 in the A549 cells transfected with circFLNA and mimic were detected by qRT-PCR. circ circFLNA, NC overexpression negative control, circ circFLNA, M miR-486-3p mimic, MC mimic control.