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. 2022 Jan 3;9:780801. doi: 10.3389/fpubh.2021.780801

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Copyright © 2022 Barrera-Avalos, Luraschi, Vallejos-Vidal, Mella-Torres, Hernández, Figueroa, Rioseco, Valdés, Imarai, Acuña-Castillo, Reyes-López and Sandino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Summary of the results obtained for the analysis of the 55 nasopharyngeal swab samples (NPSs) evaluated by Rapid Antigen Test (RAT). The 55 NPSs were diagnosed as positive for SARS-CoV-2 using the ORF1ab probe following a one-step strategy. From them, 40 NPSs showed no mutations for SARS-CoV-2 (black arrow) (Group 1). By contrast, in the other 15 positive samples we identified the SARS-CoV-2 variants K417N/T, E484K, and N501Y (gray arrows) (Group 2). Each one of these groups were used to evaluate the sensitivity of the Rapid Antigen Test (RAT) according to the Cq ranges previously obtained by RT-qPCR. The group 1 showed a 90% of positive diagnosis using the RAT. However, the group 2 showed only a 42.8% of positive diagnosis. Importantly, all of them were grouped in the 20 ≤ Cq <25 interval.