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. 2022 Jan 3;11:797007. doi: 10.3389/fonc.2021.797007

Table 1.

Sequences and parameters of primer used for qPCR.

Name Sequence GC content (%) Annealing temperature (°C)
KLF9 forward 5′-TGGCTGTGGGAAAGTCTATGG-3′ 52.4 60
KLF9 reverse 5′-CTCGTCTGAGCGGGAGAACT-3′ 60 60
TPD52 forward 5′-GCTGCTTTTTCGTCTGTTGGCT-3′ 50 60
TPD52 reverse 3′-TCAAATGATTTAAAAGTTGGGGAGTT 30 60
miR223 forward 5′-AGCCGTGTCAGTTTGTCAAAT-3′ 42.9 60
miR-223 reverse 5′-GTGCAGGGTCCGAGG TC-3′ 70.6 60
PKCϵ forward 5′-AGCCTCGTTCACGGTTCT-3′ 55.6 60
PKCϵ reverse 5′-TGTCCAGCCATCATCTCG-3′ 55.6 60