Collagen gel mixture

Reagent	Final concentration	Amount
Rat tail collagen I (3 mg/mL)	1 mg/mL	33 μL
DMEM low glucose 10×	1×	5 μL
NaOH 1M	5 μL every 100 μL of collagen	1.7/2 μL
Cells + Medium	n/a	60 μL
Total	n/a	100 μL