Collagen “adhesive” solution

Reagent	Final concentration	Amount
Rat tail collagen I (3 mg/mL)	1 mg/mL	100 μL
DMEM low glucose 10×	1×	30 μL
dH2O	n/a	165 μL
NaOH 1M	5 μL every 100 μL of collagen	5 μL
Total	n/a	300 μL