FIG. 3.

(A) The scheme of PCR mutagenesis for production of an Ag1V1/Ag2 chimeric gene. Details of the PCR mutagenesis to obtain Ag1V1/Ag2 chimeric gene are described in Materials and Methods. (B) Analysis of the resultant PCR products in a 2.0% agarose gel. Molecular size markers are indicated on the left. Lane 1, first PCR product of the Ag1V1 gene; lane 2, first PCR product of the Ag2 gene; lane 3, second PCR product of the Ag1V1/Ag2 chimeric gene. (C) Amino acid sequence of Ag1V1/Ag2 chimeric protein. The underlined amino acids were derived from an Ag1V1 (²⁰E to ⁷⁸C) clone, and the remains were derived from an Ag2 (²⁰K to ⁸⁵A) clone.