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. 2022 Jan 3;12:615253. doi: 10.3389/fpls.2021.615253

TABLE 1.

Summary of illumination programs and the corresponding physiological questions investigated by these approaches.

Abbreviation Illumination program Physiological state in question
Control
Dark 14 d of growth in 16 h WL/8 h dark; harvest under safe green
light before beginning of next light period
State of PS at the end of a dark period in a standard dark-light growth
schedule (state 1 as in Wientjes et al., 2013).
Light-quality shifts
Dark - PSII 50 14 d of growth in 16 h WL/8 h dark + 50 min PSII-light Induction of state 2 by 50 min of PSII-light instead of WL.
Dark - PSII 50 - PSI 30 14 d of growth in 16 h WL/8 h Dark + 50 min
PSII-light + 30 min PSI-light
Induction of state 1 by additional 30 min of PSI-light.
Dark-white light (WL) shifts
Dark - WL 50 14 d of growth in 16 h WL/8 h dark + 50 min WL State of PS after 50 min of WL following the last dark period (state 2 as
in Wientjes et al., 2013).
Dark - WL 50 - Dark 15 14 d of growth in 16 h WL/8 h dark + 50 min WL + 15 min dark Effect of additional 15 min of dark after 50 min illumination (re-opening
of PSII centers).
Dark - WL 2 h 14 d of growth in 16 h WL/8 h dark + 2 h WL Approval of state 2 after extended illumination with WL.
Dark - WL 2 h - Dark 15 min 14 d of growth in 16 h WL/8 h dark + 2 h WL + 15 min dark Effect of additional 15 min of dark after extended WL acclimation
(re-opening of PSII centers).

All plants analyzed were 14 days old. For plants subjected to long-term light-quality treatments the pre-growth phase was correspondingly shortened. Abbreviations used: d, days; h, hours; min, minutes; PS, photosynthesis apparatus. Abbreviations for light sources are as given in main text.