TABLE 1.
Abbreviation | Illumination program | Physiological state in question |
Control | ||
Dark | 14 d of growth in 16 h WL/8 h dark; harvest under safe green light before beginning of next light period |
State of PS at the end of a dark period in a standard dark-light growth schedule (state 1 as in Wientjes et al., 2013). |
Light-quality shifts | ||
Dark - PSII 50 | 14 d of growth in 16 h WL/8 h dark + 50 min PSII-light | Induction of state 2 by 50 min of PSII-light instead of WL. |
Dark - PSII 50 - PSI 30 | 14 d of growth in 16 h WL/8 h Dark + 50 min PSII-light + 30 min PSI-light |
Induction of state 1 by additional 30 min of PSI-light. |
Dark-white light (WL) shifts | ||
Dark - WL 50 | 14 d of growth in 16 h WL/8 h dark + 50 min WL | State of PS after 50 min of WL following the last dark period (state 2 as in Wientjes et al., 2013). |
Dark - WL 50 - Dark 15 | 14 d of growth in 16 h WL/8 h dark + 50 min WL + 15 min dark | Effect of additional 15 min of dark after 50 min illumination (re-opening of PSII centers). |
Dark - WL 2 h | 14 d of growth in 16 h WL/8 h dark + 2 h WL | Approval of state 2 after extended illumination with WL. |
Dark - WL 2 h - Dark 15 min | 14 d of growth in 16 h WL/8 h dark + 2 h WL + 15 min dark | Effect of additional 15 min of dark after extended WL acclimation (re-opening of PSII centers). |
All plants analyzed were 14 days old. For plants subjected to long-term light-quality treatments the pre-growth phase was correspondingly shortened. Abbreviations used: d, days; h, hours; min, minutes; PS, photosynthesis apparatus. Abbreviations for light sources are as given in main text.