TABLE 2.
Set-up | Fv/Fm | qT1 | qT1 approx | qT2 | qT2 approx | F r | ΔFr |
Internal LED lights |
WT: 0.76 ± 0.01 stn7: 0.79 ± 0.01 |
WT: 0.028 ± 0.008 stn7: −0.025 ± 0.002 |
0.053 ± 0.007 |
WT: 0.049 ± 0.022 stn7: −0.032 ± 0.003 |
0.082 ± 0.019 |
WT: 0.67 ± 0.05 stn7: −0.2 ± 0.02 |
0.87 ± 0.087 |
PSII-/PSI-light system |
WT: 0.81 ± 0.00 stn7: 0.77 ± 0.03 |
WT: 0.04 ± 0.02 stn7: 0.01 ± 0.01 |
0.05 ± 0.071 |
WT: 0.07 ± 0.013 stn7: 0.027 ± 0.021 |
0.047 ± 0.034 |
WT: 0.47 ± 0.2 stn7: 0.04 ± 0.08 |
0.43 ± 0.11 |
Dark/white- light system |
WT: 0.82 ± 0.00 stn7: 0.77 ± 0.01 |
WT: 0.101 ± 0.014 stn7: 0.044 ± 0.024 |
0.057 ± 0.025 |
WT: 0.166 ± 0.036 stn7: 0.064 ± 0.017 |
0.102 ± 0.036 |
WT: 0.9 ± 0.02 stn7: 0.6 ± 0.03 |
0.3 ± 0.066 |
Calculation of Chl fluorescence parameters was done with data obtained in experiments shown in Figure 1. Results are means of three independent biological replicates. Standard deviations are given. Calculation of Fv/Fm: Fm − F0/Fm. Calculation of qT (state transition dependent quenching) with the saturation pulse method was done separately for the transitions from state 2 to state 1 (qT1) and from state 1 to state 2 (qT2) as: qT1 = (Fm1 − Fm2)/Fm1 and qT2 = (Fm1 − Fm2′)/Fm1; with Fm1, Fm2 and Fm2′ as maximum fluorescence yields obtained in stable state 1 and 2, respectively (compare Supplementary Figure S1). Approximation of state transitions in the light quality and dark-light shift systems is calculated as: qTn approx = qTn WT–qTn stn7. Calculation of Fr according to Haldrup et al. (2001): Fr = [(Fi′ − Fi) − (Fii′ − Fii)]/(Fi′ − Fi) with Fi and Fii as fluorescence yield in state 1 or 2, and with Fi′ and Fii′ as fluorescence yield in state 1 or 2 directly after illumination shift. Approximation of state transitions on base of Fr in WT and stn7 is given as: ΔFr = Fr WT − Fr stn7.