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. 2022 Jan 3;12:804813. doi: 10.3389/fmicb.2021.804813

Figure 5.

Figure 5

The effects of WT-exosomes and miR-223-KO exosomes on cecal ligation and puncture (CLP)-induced inflammatory response, cardiac dysfunction and animal mortality. (A–C) CLP-mice treated with WT-exosomes (n = 11) showed lower levels of serum TNF-α (A), IL-1β (B), and IL-6 (C), whereas CLP-mice injected with KO-exosomes (n = 11) exhibited higher levels of circulating TNF-α (A), IL-1β (B), and IL-6 (C), compared with those treated with incomplete DMEM medium (n = 10; ^p < 0.05 vs. shams; *p < 0.05 vs. CLP + medium; #p < 0.05 vs. CLP + medium). (D) Results of echocardiography measurement showed that values of the left ventricular ejection fraction (EF%, E) and the fractional shortening (FS%, F) were significantly decreased in CLP mice injected with incomplete DMEM medium (n = 10), compared with shams (n = 8). Remarkably, the reduction of EF% and FS% was attenuated in WT-exosome-treated CLP mice (n = 11); whereas it was aggravated in CLP mice administrated with miR-223-KO exosomes (n = 11; ^p < 0.05 vs. shams; *p < 0.05 vs. CLP + medium; #p < 0.05 vs. CLP + medium). (G) The survival of CLP-mice was significantly improved by WT-exosome treatment, whereas it was worse by miR-223-KO exosome injection (n = 8, *p < 0.05 vs. CLP + medium; Adopted from (Wang et al., 2015) distributed under the creative commons CC BY license).