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. 2022 Jan 3;12:811364. doi: 10.3389/fimmu.2021.811364

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Copyright © 2022 Zhang, Zhu, Zheng, Wang, Li, Feng, Shang, He, Jiang, Shi, Wang, Cao and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction and expression of anti-CD19 CAR. (A) The construction of anti-CD19 CAR. (B) The retroviral vector copies detected by RT-qPCR (n=3 donors). (C) Transduction efficiency of human T cells with anti-CD19 CAR retroviral vectors measured by flow cytometry analysis (n=6 donors). (D) Representative flow cytometry analysis profiles of transduction efficiency in an independent donor out of 6 donors. (E) The vector copy number integration per T cell determined by RT-qPCR (n=3 donors). (F) The expression of miR155 detected by TaqMan^® Assay-based RT-qPCR at the 6th, 8th,10th, 14th day after T cell isolation (n=3 donors). (G) The expression of LSD1 detected by SYBR Green-based RT-qPCR at the 6th, 8th,10th, 14th day after T cell isolation (n=3 donors). (F, G) Values were expressed as the means ± SD. Unpaired t test was performed. **p<0.01; *p<0.05; compared to RNAU6 anti-CD19 CAR-T cells.