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. 2022 Jan 3;12:811364. doi: 10.3389/fimmu.2021.811364

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Copyright © 2022 Zhang, Zhu, Zheng, Wang, Li, Feng, Shang, He, Jiang, Shi, Wang, Cao and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RNA sequencing revealed the miR155 targets in anti-CD19 CAR-T cells. (A) Volcano map of differential expression. (B) Cluster heat map of gene expression. (C) Predicted targets of miR155 in miR155 over-expressing anti-CD19 CAR-T cells. The Venn diagram showed the overlap between gene downregulated in miR155 over-expressing anti-CD19 CAR-T cells and predicted targets of miR155 in TargetScan dataset. (D) The cluster heat map of differential gene expression. (E) The verification of differential expression by RT-qPCR. Values were expressed as the means ± SD. Unpaired t test was performed. ***p<0.001, **p<0.01, *p<0.05 compared to RNAU6 anti-CD19 CAR-T cells.