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. 2022 Jan 3;12:811364. doi: 10.3389/fimmu.2021.811364

Figure 6.

Figure 6

RNA sequencing revealed the target genes regulated by LSD1 in anti-CD19 CAR-T cells. (A) Volcano map of differential expression between LSD1 shRNA-1 co-expressing anti-CD19 CAR-T cells and RNAU6 anti-CD19 CAR-T cells. (B) Cluster heat map of gene expression in LSD1 shRNA-1 co-expressing anti-CD19 CAR-T cells and RNAU6 anti-CD19 CAR-T cells. (C) Volcano map of differential expression between LSD1 shRNA-2 co-expressing anti-CD19 CAR-T cells and RNAU6 anti-CD19 CAR-T cells. (D) Cluster heat map of gene expression in LSD1 shRNA-2 co-expressing anti-CD19 CAR-T cells and RNAU6 anti-CD19 CAR-T cells. (E) The Venn diagram shown the overlap between gene upregulated or downregulated in LSD1 shRNA-1 anti-CD19 CAR-T cells and LSD1 shRNA-2 anti-CD19 CAR-T cells. (F) Network of protein interaction analysis with the 212 up-expressing genes and 264 down-expressing genes in (E). Red-colored ones associated with the term of cell cycle, purple-colored ones associated with the term of regulation of cell cycle, and green-colored ones associated with the term of immune effector process. (G) The verification of differential expression by RT-qPCR. Values were expressed as the means ± SD. Unpaired t test was performed. ***p<0.001, **p<0.01, *p<0.05 compared to RNAU6 anti-CD19 CAR-T cells.