Figure 4.

The expression levels of known adenoviral receptors and coreceptors in the established cell lines and the short-term primary cultures did not predict the relative oncolytic efficiency of the fiber-modified recombinant adenoviruses

The oncolytic efficacy represented by the IC50 values (vp/cell) for the indicated rAds (left panel) and the surface expression of receptors/coreceptors (percentage of positive cells, right panel) analyzed by flow cytometry are plotted as a heatmap. The IC50 values of rAds for established glioma cell lines and short-term primary glioma or normal cultures were derived from the resazurin/Alamar Blue cell viability assays 5 (except T98G, 7 days) and 7 days (except GliBah, 6 days) post-infection, respectively. Therefore, the three scale bars of the IC50 values are shown reflecting different readout time points in the cell viability assays. See also Figures S6–S8 and Table S3 for flow cytometry plots and statistics.