Figure 5.
The comparative cytotoxic efficacy of the fiber-modified replication-competent recombinant adenoviruses in rodent glioma and carcinoma cell lines
(A–F) Crystal violet cell viability assays of rAd-infected cells 5 days post-infection. Cells (2.5 × 104/well) were infected in suspension with a serial dilution of the indicated rAds. Replication-defective Ad5ΔE1A was used as a negative control. Representative pictures of three independent experiments in technical duplicates with similar results are shown. (A) GL261 murine glioma cells. (B) CT-2A murine glioma cells. (C) C6 rat glioma cells. (D) 4T1 murine mammary carcinoma cells. (E) CT26 murine colon carcinoma cells. (F) B16-F10 murine melanoma cells. (G–I) Resazurin/Alamar Blue cell viability assays of rAd-infected cells 5 days post-infection. Cells (2.5 × 103/well) were infected in suspension with a serial 3-fold dilution of the indicated rAds starting from 20,000 vp/cell. Normalized data are presented as the mean ± SD of three independent experiments in technical triplicates. See also Figure S9.