FIG. 1.

M. avium subsp. paratuberculosis (M. paratub.) and M. avium subsp. avium (M. avium) CF and sonicate proteins were separated in SDS-PAGE and blotted onto nitrocellulose membranes. The amounts of total protein applied were 1 μg for lanes A and B and 5 μg for lanes C. The molecular masses (in kilodaltons) are indicated. The two lanes A were incubated with antiserum against M. avium subsp. avium CF, and the two lanes B were incubated with antiserum against M. avium subsp. paratuberculosis CF. The four lanes C were incubated with antiserum against M. avium subsp. avium sonicate (Dako).