FIG. 5.

SDS-PAGE with CBB staining and Western blot of sonicate (Son) and CF antigens of various mycobacterial species. The molecular masses (in kilodaltons) of the standard (St) are indicated. G, proteins in gel stained with CBB; B, lanes blotted onto nitrocellulose and incubated with antiserum against the 14-kDa protein isolated from M. avium subsp. paratuberculosis; MP2E, M. avium subsp. paratuberculosis strain 2E; M.s., M. scrofulaceum; M.t., M. tuberculosis; M.f., M. fortuitum; M.k., M. kansasii; M.ph, M. phlei; M.i, M. intracellulare.