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. 2021 Nov 29;81(2):214–224. doi: 10.1136/annrheumdis-2021-220875

Figure 2.

Figure 2

Yap, Snail and Ctgf are upregulated in inflammatory arthritis in mice. (A–C) Immunohistochemical detection of Yap (A), Snail (B) and Ctgf (C) in mouse synovium 6 days after AIA induction (n=5; 2 male mice, 3 female mice, 11–13 weeks). Contralateral knee served as control. Haematoxylin counterstain is shown in blue. Boxed areas on the left are shown at higher magnification on the right. Arrow (A) indicates Yap-expressing cells penetrating through the bone into the underlying marrow space. Scale bars: 20 µm. For isotype negative control stainings, see online supplemental figure 10f–h. Graphs indicate protein expression in synovium based on IHC staining intensity, with lines and error bars indicating mean±SD (n=5). P values indicate statistical significance using an unpaired two-tailed t-test. AIA, antigen-induced arthritis; A, articular cartilage; B, bone; Ctgf, connective tissue growth factor; S, synovium; Yap, Yes-associated protein.