Figure 1.

Effect of arsenic stress on the expression of microglial CD200R1. BALB/c mice were exposed to sodium arsenite (arsenic) for 2 months and checked the expression of CD200R1 in the whole brain (n = 4 mice/group). A, Western blots, (B) immunostaining of CD200R1 in brain section. Scale bar: 50 μm for uncropped images and 11 μm for the cropped images. Mouse primary neonatal microglia were treated with arsenic as well as LPS in vitro for 72 h, and CD200R1 expression was checked (n = 4). C, Western blot, (D) immunostaining of CD200R1 in mouse primary neonatal microglia. Scale bar: 10 μm. The levels of mRNA of (E) CD200R1 were measured 72 h following treatment with arsenic and LPS in primary microglia and, (F) CEBPβ was measured (n = 3) in primary microglia 2 h following treatment with arsenic and LPS. Another set of cells was treated in vitro with arsenic, and Taqman Low-Density Array (TLDA) was run to detect the changes in the global miRNA profile (n = 2). G, fold change in the miRNA level was presented as a heatmap, (H) miRNA upregulated more than 1.5-fold and downregulated more than 0.8-fold was presented in the table; (I) Network generated by IPA with the up- and downregulated genes and miRNA in arsenic-treated microglia indicated a possible relationship between CD200R1 and miR-129-5p. The symbols used in the network map are shown in the legend. “n” denotes the number of independent study for in vitro experiments. Bar graphs represent mean ± SEM. “p” denotes the level of significance in comparison to control; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ns, nonsignificant.