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. 2021 Dec 22;298(1):101521. doi: 10.1016/j.jbc.2021.101521

Figure 4.

Figure 4

Effect of arsenic on the methylation status of the miR-129-2 promoter and DNMT1 expression in primary neonatal microglia.A, in silico analysis, using MethPrimer software, predicted 15 CpG sites in the promoter region of miR-129-5p (bp 412−bp 832). B, bisulfite sequence of the promoter region of miR-129-5p was performed, and a methylation map of the CpG sites was generated using the MethPrimer software. C, the methylation status was quantitated and represented in a histogram (n = 4 samples/group). D, arsenic treatment for 72 h reduced the expression of DNMT1 in primary microglia, which is shown by representative Western blot and its densitometric analysis presented as a histogram (n = 3). “n” denotes the number of independent study for in vitro experiments. Bar graphs represent mean ± SEM. “p” denotes the level of significance in comparison to control; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ns, nonsignificant.