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. 2021 Dec 22;298(1):101521. doi: 10.1016/j.jbc.2021.101521

Figure 6.

Figure 6

Expression of CD200R1, miR-129-5p, DNMT1, and Cytokines in human blood. PBMCs were isolated from the blood of unexposed (n = 5 individuals) and arsenic-exposed symptomatic individuals (n = 9 individuals). RNA was isolated, and the levels of (A) CD200R1 mRNA and (B) miR-129-5p were measured by real-time PCR. The expression levels of CD200R1 and DNMT1 were checked by Western blot analysis in PBMC lysate, (C) image of the Western blot and quantitative analysis of the expression of (D) CD200R1 and (E) DNMT1. Pearson correlation analysis revealed an inverse correlation between (F) miR-129-5p and CD200R1 as well as (G) miR-129-5p and DNMT1, whereas (H) CD200R1 and DNMT1 were directly correlated. “R” denotes Pearson correlation coefficient. For cytokine analysis, RNA was isolated from the whole blood of unexposed and arsenic-exposed individual followed by qRT-PCR for cytokines (n = 4–5 individuals/group). The level of mRNA was present as scatter plot for (I) TNF- α and (J) IL-6. An increased pattern in the level of IL-6/TNF-α mRNA was observed in arsenic-exposed individuals. Bar graphs represent mean ± SEM. “p” denotes the level of significance in comparison to control; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ns, nonsignificant.