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. 2022 Jan 3;12:760416. doi: 10.3389/fgene.2021.760416

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Copyright © 2022 Tao, Yang, Zhang, Zhang, Suo, Li, Liu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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XR_311113.2 serves as a sponge for chi-miR-424-5p. (A) Alignment of potential chi-miR-135a, chi-miR-424-5p, chi-miR-544-5p, and chi-miR-3955-5p base pairing with XR_311113.2, as identified by the miRanda software. (B) The luciferase activity of miRNA binding sites mutant pmirGLO vector by luciferase assay in GK cells. Site-directed mutagenesis in the chi-miR-135a, chi-miR-424-5p, chi-miR-544-5p, and chi-miR-3955-5p binding site of the XR_311113.2. The pmirGLO-1 and pmirGLO-2 were wild vector of XR_311113.2. (C) Luciferase reporter assay to determine the luciferase activity of pmirGLO-2 in GK cells transfected with chi-miR-424-5p and chi-miR-3955-5p mimics, to identify miRNAs that bound to the XR_311113.2 sequence. **p < 0.01; ***p < 0.001; NS, not significant.