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. 2022 Jan 3;12:805827. doi: 10.3389/fmicb.2021.805827

Figure 2.

Figure 2

Apigenin ameliorates HFD-induced systemic inflammation, steatohepatitis, and adipose tissue inflammation in mice. (A,B) Serum levels of ALT and AST. (C,D) Serum levels of TNF-α and MCP-1. (E) The gross appearance of the liver. Liver lipid accumulation was assessed by HE and ORO staining, scale bar, FIGURE 2 | 100 μm. Immunofluorescence staining for F4/80 in liver tissue, scale bar, 50 μm. The gross appearance of the Epi-WAT. HE staining of Epi-WAT, scale bar, 100 μm. (F) NAFLD activity score. (G) Quantification of ORO staining. (H) Quantification of F4/80+ staining. (I) Quantification of adipocyte size. (J) Relative expression of TNF-α, IL-1β, IL-10, and MCP-1 in hepatic was assessed using qRT-PCR and in comparison with the ND group. (K) Relative expression of TNF-α, IL-1β, IL-10, and MCP-1 in Epi-WAT was assessed using qRT-PCR and in comparison with the ND group. Error bars are expressed as means ± SEM (n = 6). Statistical significance was determined by one-way ANOVA with Tukey tests for multiple-group comparisons. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. ND; #p < 0.05 ##p < 0.01, and ###p < 0.001 vs. HFD.