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. 2022 Jan 3;12:725186. doi: 10.3389/fphar.2021.725186

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Copyright © 2022 Zhong, Chen, Li, Chen, Qian, Chen, Wang and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dangshen Erling decoction (DSELD) protects from myocardial hypertrophy and mitochondrial permeability transition pore (mPTP) in the conditioned media (CM)-induced inflammation in H9C2 cells. (A) The operation mode diagram of the RAW264.7 and H9C2 cells. (B) RAW264.7 cells were incubated with lipopolysaccharide (LPS; 1 μg/ml) with or without DSELD (50 μg/ml) for 24 h, and nitric oxide (NO) secretion was detected in cell supernatant by ELISA. (C) Representative fluorescent images of cardiomyocytes from the different groups [green: phalloidin, red: tetramethyl rhodamine methyl ester (TMRM), and blue: DAPI]. (D) The relative area was corrected by the nucleus and normalized to control (F-actin, ×400). (E) The fluorescence intensity to control (TMRM, ×200). *p < 0.05, **p < 0.01.