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. 2022 Jan 3;12:764130. doi: 10.3389/fphar.2021.764130

FIGURE 6.

FIGURE 6

Rb2 attenuated miR-216a–induced lipid accumulation in foam cells. (A) Photomicrographs of lipid accumulation within the cytoplasm (red staining) with or without Rb2 treatment in THP-1–derived foam cells transfected with miR-216a mimics. (B) Quantification of the percentage of Oil red O–positive area (of the total cell area) with or without Rb2 treatment in THP-1–derived foam cells transfected with miR-216a mimics. (C) The mRNA expression of lipid uptake–related genes CD36 and SR-A1 with or without Rb2 treatment in THP-1–derived foam cells transfected with miR-216a mimics. (D) The cholesterol efflux ability with or without Rb2 treatment in THP-1-derived macrophages transfected with miR-216a mimics. (E) The mRNA expression of cholesterol efflux gene ABCG1 with or without Rb2 treatment in THP-1–derived foam cells transfected with miR-216a mimics. (F) Photomicrographs of lipid accumulation within the cytoplasm (red staining) with or without Rb2 treatment in the human PBMC–derived foam cells transfected with miR-216a mimics. (G) Quantification of the percentage of Oil red O–positive area (of the total cell area) with or without Rb2 treatment in the human PBMC-derived foam cells transfected with miR-216a mimics. *p < 0.05, **p < 0.01, compared to the NC group. & p < 0.05, # p < 0.05, ## p < 0.01, compared to the miR-216a group. n = 5 for each group. Scale bar = 50 μM.