Figure 5.

DHA-TF adducts enhance TRAIL efficacy in TRAIL-sensitive TNBC cell lines. (A) The sensitivity of TNBC cell lines to TRAIL-induced apoptosis. All cell lines were treated with different concentration of rhTRAIL WT for 24 h. (B) All cell lines were pre-treated with DHA or DHA-TF for 30 min, followed with 5 ng/mL rhTRAIL WT, 4C7, or DHER for 24 h. Individual treatment of DHA is shown in grey, and individual treatment of DHA-TF is shown in black. Cell viability was determined by MTS assay. Data shown are mean ± SEM from three independent experiments performed in triplicate. *0.01 < p ≤ 0.05, **0.001 < p ≤ 0.01, ***0.0001 < p ≤ 0.001, ****p ≤ 0.0001.