Figure 1.

Intravital imaging of the immune system under inflammatory conditions. (A) Representation of the surgical model used to perform intravital imaging in the murine popliteal lymph node, including a minimally invasive surgery and imaging through a transparent window. (B) Example of intravital imaging setup based on 2-photon microscopy, including a pulsed laser with near-infrared (NIR) emission wavelengths and photomultipliers (PMT) for fluorescence detection. (C) 4D videos (3D z-stacks over time) capturing cell motility are acquired and visualized on a computer. Cells are tracked (white lines) to compute metrics such as speed, directionality (dir), and plotting of tracks with a common origin (PTCO).