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. 2022 Jan 3;12:801730. doi: 10.3389/fphar.2021.801730

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Copyright © 2022 Li, Yang, Lin, Yan, Deng, Ye, Shi, Wu, Ma, Xu, Tan, Gao and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Limonin reduced lipid accumulation caused by TAA in zebrafish liver. (A) Oil red O staining of frozen sections of zebrafish larvae. (B) Frozen liver sections of zebrafish larvae with liver-specific eGFP expression were stained with Nile Red. Figures are magnified as ×200. (C) Quantitative analysis of the area of lipid droplet in liver based on Oil Red O staining. (D) Quantitative analysis of the liver mean fluorescence intensity of Nile Red. (E) Real-time PCR analysis of FASN mRNA levels in zebrafish larvae. The mRNA levels were normalized to β-actin mRNA levels and presented as fold change compared with the control group. Data are shown as the mean ± SD (n = 6–8 per group). #p < 0.05, ##p < 0.01, ###p < 0.001 vs control group; *p < 0.05, **p < 0.01, ***p < 0.001 vs TAA group.