Skip to main content
. 2022 Jan 3;12:750261. doi: 10.3389/fendo.2021.750261

Figure 3.

Figure 3

Dual-luciferase reporter assay for Ddit4 3’UTR and expression of Ddit4 in mice. (A) Dual-luciferase reporter assay using pmirGLO-Ddit4 WT 3’-UTR, pmirGLO-Ddit4 MT 3’-UTR, and pmirGLO no-insert control plasmids. 3T3-L1 cells were co-transfected with Syn-mmu-miR-221-3p (n=4), Syn-mmu-miR-222-3p (n=4), negative control siRNA (n=8). (ns, not significant). (B) Quantitative RT-PCR for Ddit4 in epididymal adipose tissues of Mir221/222flox/y (n=4) and Mir221/222AdipoKO (n=8) mice fed with high fat high sucrose (HFHS). Western blot analyses for DDIT4 and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) in epididymal adipose tissues of Mir221/222flox/y (n=3) and Mir221/222AdipoKO (n=7) mice fed with high fat high sucrose (HFHS). Data shown as mean ± SD and analyzed by Mann-Whitney’s U test (*p<0.05; **p<0.01).