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. 2022 Jan 3;12:750261. doi: 10.3389/fendo.2021.750261

Figure 4.

Figure 4

Western blot analyses. (A) Western blot analyses for AKT (protein kinase B), TSC2 (tuberous sclerosis complex 2), mTOR (mammalian target of rapamycin) and S6K (ribosomal protein S6 kinase) in Mir221/222flox/y (n=3) and in epididymal adipose tissues of Mir221/222AdipoKO (n=5) male mice fed with high fat high sucrose (HFHS). Activated form of p-AKT and Inactivated form of p-TSC2 (Thr-1462) and activated form of p-S6K are shown. (B) Densitometric analyses of Western blots. Data shown as mean ± SD and analyzed by Mann-Whitney’s U test (*p<0.05).