Figure 4.
Western blot analyses. (A) Western blot analyses for AKT (protein kinase B), TSC2 (tuberous sclerosis complex 2), mTOR (mammalian target of rapamycin) and S6K (ribosomal protein S6 kinase) in Mir221/222flox/y (n=3) and in epididymal adipose tissues of Mir221/222AdipoKO (n=5) male mice fed with high fat high sucrose (HFHS). Activated form of p-AKT and Inactivated form of p-TSC2 (Thr-1462) and activated form of p-S6K are shown. (B) Densitometric analyses of Western blots. Data shown as mean ± SD and analyzed by Mann-Whitney’s U test (*p<0.05).