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. 2022 Jan 3;12:750261. doi: 10.3389/fendo.2021.750261

Figure 5.

Figure 5

Adipogenesis, lipogenesis and lipolysis in 3T3-L1 cells. Lentiviral vectors were transduced to 3T3-L1 cells at 2 days before the induction of differentiation and cultured for 7 days. (A) Oil-red O staining of differentiated 3T3-L1 cells treated with pLV-locker control, pLV-locker 221, pLV-locker 222, and pLV-locker 221/222. (B) Oil-red O staining of differentiated 3T3-L1 cells treated with pVL control, pLV 221, pLV 222, and pLV221/222. (C) Western blot analysis of PPARγ in differentiated 3T3-L1 cells treated with pLV-locker control, pLV-locker 221, pLV-locker 222, and pLV-locker 221/222. (D) Western blot analysis of PPARγ in differentiated 3T3-L1 cells treated with pVL control, pLV 221, pLV 222, and pLV221/222. (E) Lipolysis assay in 3T3-L1 cells stimulated with isoproterenol. Differentiated 3T3-L1 cells were treated with pLV-locker 221/222 and pLV 221/222. Data shown as mean ± SD and analyzed by one-way ANOVA with Tukey test (*p<0.05; **p<0.01).