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. 2022 Jan 3;12:763392. doi: 10.3389/fendo.2021.763392

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Copyright © 2022 Busby, Chen, Godfrey, Rehan, Wildman, Smith and Hassan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PBAF complex is critical for early differentiation and matrix maturation. (A) Quantitative Western blot analysis of PBAF factors from primary rat calvarial osteoblast cells induced to differentiate for 20 days. (B) Relative mRNA levels of PBAF complex, obtained by real-time RT-qPCR using DNase I treated total RNA isolated from murine calvarial osteoblast cells induced to differentiate for 20 days. (C-H) Histone H3K9 and H3K27 modifications on the promoter regions of PBAF complex members and Runx2 during Bone Marrow Stromal Cells (BMSCs) differentiation. Illumina ChIP-seq genomic tracks at (C) Pbrm1 (Baf180), (D) Arid2 (Baf200), (E) Brd7, (F) Brg1, (G) Smarcc1 (Baf155) and (H) Runx2 promoter regions. Quantitative peaks representing active chromatin marks H3K9ac and H3K27ac, as well as peaks representing repressive chromatin marks H3K9me3 and H3K27me3.