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. 2022 Jan 3;12:763392. doi: 10.3389/fendo.2021.763392

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Copyright © 2022 Busby, Chen, Godfrey, Rehan, Wildman, Smith and Hassan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The shRNA mediated knockdown of Baf45a in odontoblast OD-21 cells affect growth, DNA repair, and apoptosis. (A) Volcano plot comparison of differential gene expression between control shRNA (non-specific) and Baf45a-specific shRNA. Red dots highlight upregulated, and green dots highlight downregulated differentially expressed genes with threshold FDR < 0.01 and Log2 Fold Change [≥ 1]. The blue zone indicates the number of transcripts that do not show significant differential expression. (B) Functional annotation based on Gene Ontology (GO) categorization. The horizontal bar plot represents differentially enriched genes linked to biological processes (FDR < 0.05) identified in control shRNA vs. Baf45a specific shRNA. *P < 0.05. (C–E). Heatmap of differentially expressed genes belongs to DNA replication, apoptosis, and cell cycle. (F) The relative changes in the expression of osteoblast and odontoblast-specific transcription factors and markers genes due to Baf45a knockdown.