Figure 7.

Real-time PCR analysis of SCs functionality. Gene expression of AMH (A), and inhibin B (B) in SCs at 24 h and 1, 2, and 3 weeks of incubation with TiO₂ NPs 5 (black bar) and 100 µg/ml (grey bar). ELISA assay of (C) AMH and (D) inhibin B secretion in SCs at 24 h and 1, 2, and 3 weeks of incubation with TiO₂ NPs 5 (black bar) and 100 µg/ml (grey bar). Data represent the mean ± SEM (^* p < 0.05 and ^** p < 0.001 vs. unexposed SCs (0 TiO₂ NPs, black dotted line) of three independent experiments, each performed in triplicate).