Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 3;9:806982. doi: 10.3389/fcell.2021.806982

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Binner, Kogan, Panser, Schleiffer, Deneke and Pauli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Identification of Spaca6 in zebrafish. (A). Expression and genomic features of zebrafish spaca6. Coverage tracks for RNA sequencing data from oocyte and testis aligned with ENSEMBL (ENSDART00000155083.2) and NCBI (XM_021466914.1) annotations as well as the full-length, newly identified testis coding DNA sequence (correct CDS). Genomic coordinates are based on GRCz11. (B). Protein domain structure of full-length Spaca6 and sequence alignment of the mature extracellular region of selected vertebrate Spaca6 proteins. Secondary structure prediction of the zebrafish Spaca6 protein sequence is depicted above the sequence alignment (coil: alpha-helix; arrow: beta-sheet). The extracellular domain (ECD) is shown in blue, while the Immunoglobulin-like domain (IG) is shown in orange. SP, signal peptide; TMD, Transmembrane domain; CD, Cytoplasmic domain. (C). Tertiary structure predictions of mouse and zebrafish Spaca6. Protein folding predictions of mature Spaca6 (lacking the signal peptide sequence) were performed using AlphaFold2 (Jumper et al., 2021). Extracellular domain, blue; Ig-like domain, orange; Transmembrane domain, green; Cytoplasmic domain, yellow. (D). Taxonomic tree of DC-STAMP-like proteins, Izumo1 and Spaca6 across vertebrates and invertebrates. DC-STAMP-like proteins (blue star) are conserved both in vertebrates and invertebrates; Izumo1 (orange circle) and Spaca6 (green square) are conserved only in vertebrates.