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. 2022 Jan 3;15:725195. doi: 10.3389/fncel.2021.725195

Figure 4.

Figure 4

In vivo delivery of SMN-OPC cell suspensions into the contusion cavity and survival at 10 days post-transplantation. (A) Schematic overview of the SMN-OPC cell suspension grafts. Pink depicts spinal motor neurons, purple depicts spinal interneurons that co-differentiate, and green is GFP-OPCs. (B) Schematic overview of SMN-OPC cervical hemicontusion grafting experiment (N = 6 animals). *Data provided in this figure are from the 10 days time point. (C) STEM121/GFP-OPC/DAPI merged image at the 10 days time point. (D) TUJ1 immunostain validates retention of the neuronal phenotype since this antibody detects the neuron-specific β-tubulin isotype, β-III-tubulin. (E) High magnification image of suspension SMN and GFP-OPC survival 10 days post-transplantation. SYN1 co-localization with STEM121 is shown. Low magnification STEM121/GFP-OPC image denotes position of field acquired caudal to the lesion epicenter (white box). R (rostral), C (caudal). Individual scale bars are provided.